Vanderbilt University researchers have designed a system for non-invasive discrimination between normal and cancerous skin lesions. The system combines the depth-resolving capabilities of OCT technique with Raman Spectroscopy’s specificity of molecular chemistry. By linking both imagining techniques into a single detector arm, the complexity, cost, and size of previously reported RS-OCT instruments have been significantly improved. The combined instrument is capable of acquiring data sets that allow for more thorough assessment of a sample than existing optical techniques.
Raman spectroscopy excels at label-free detection of a sample’s biochemical composition but is incapable of high-resolution microstructural mapping.
Optical coherence tomography excels at acquiring micron scale cross-sectional images but lacks inherent sensitivity to compositional properties of the sample.
Combined RS-OCT, invented and patented by the same researchers in 2009, brings together the biochemical composition detection of Raman spectroscopy with the microstructural imaging capability of optical coherence tomography.
This technology takes advantage of the unique properties of RS-OCT without the need for separate detection arms, a novel approach to RS-OCT systems. The utility of a common detector RS-OCT instrument is broad; any sample whose microstructural architecture and biochemical composition is worth evaluating can benefit from RS-OCT. Currently, a working prototype has been developed and the potential utility of the instrument in the skin, retina, and murine calvaria has been demonstrated.
In Vivo RS-OCT of a scab on the back of a finger. In the OCT image (top), the center of the scab is clearly seen as ahyperreflective (dark) region in the center of the image. The vertical lines in the OCT image depict the axis from which Raman spectra were acquired. The Raman spectrum from the center of the scab exhibits subtle differences (arrows) in spectral regions corresponding to peaks seen in collagen and keratin. CA Patil et al., Opt. Lett. 33, 1135-1137 (2008)
- Cost effective than the multi-sensor arms found in previous embodiments
- Less instrumentational complexity inherent in a single detector arm
- Smaller size of complete system
Intellectual Property Status