Sample preparation for droplet digital PCR applications

This opportunity seeks innovative solutions for a universal sample preparation method that enables accurate, consistent, and sensitive pathogen quantification across diverse food matrices, supporting droplet digital PCR applications in food safety testing.

Priority Areas

• Filtration technologies: Methods to separate target particles from larger debris
• Magnetic bead-based capture: Systems delivering ddPCR-grade purity
• Microfluidic cartridges: For standardized sample processing
• Heat-stable, low-volume prep protocols: For simple lab use
• Inhibitor-binding solutions: Membranes or additives for complex sample cleanup
• Extraction kits: Optimized for meat, dairy, and plant-based matrices
• Combined approaches: Integration of multiple technologies

Eligibility & Requirements

Must Have:

• Capable of purifying samples to yield DNA suitable for droplet digital PCR
• Able to concentrate large-volume eluates (>300 mL) to <100 µL for low-concentration quantification
• Applicable to food matrices ranging from simple rinses to complex samples (e.g., ground beef)
• Completable within a standard lab shift without overnight incubation
• Feasible for parallel sample processing or potential for future scale-up

Nice to Have:

• Processing time under three hours
• Adaptable to wide range of food matrices without significant re-optimization
• Low hands-on time and minimal number of steps
• Shelf-stable reagents or kits requiring minimal cold chain logistics
• Minimal use of hazardous solvents or alignment with green chemistry principles

Please note: Full RFP is attached in the "More Information" section of this page. Faculty and researchers interested in applying for these opportunities based on technologies developed or disclosed at Vanderbilt must submit their proposals through the CTTC.